The obscure iconography of Dosso's canvas has caused much speculation. In the past it has been titled simply Scene from a Legend and, more often, Departure of the Argonauts. The present title refers instead to an event in Virgil's Aeneid. Designed to celebrate the origin and growth of the Roman Empire, the Aeneid tells the story of Aeneas, who after the fall of Troy and seven years wandering, founded a settlement on the Italian peninsula, establishing the Roman state. The story of Aeneas and Achates is taken from Book I of the Aeneid, where Aeneas and his faithful companion Achates, their journey just begun, take refuge on the Libyan coast after their ships are wrecked in a storm.
Two other surviving scenes from the Aeneid by Dosso have been located, one in England, the other in Canada, and along with the Washington canvas have been identified as part of a frieze of ten pictures painted by the artist for the camerino, or study of Alfonso d'Este in his castle at Ferrara. Dosso Dossi was greatly influenced by Venetian art, especially the use of color and treatment of landscape as seen in works by Titian and Giorgione. He was perhaps best known in his time for soft, feathery landscapes and scenes of everyday life that are nevertheless infused with a touch of fantasy.